Description
SPARCL1 (Secreted Protein, Acidic and Rich in Cysteineslike 1), also known as hevin, SC1 or MAST9, is a member of the SPARC family of extracellular glycoproteins. SPARCL1 is an antiadhesive protein that is widely expressed in tissues such as brain, heart, lung, muscle and kidney, but not liver. Human SPARCL1 contains a 16 amino acid (aa) signal sequence and a 648 aa mature region with four domains: a 416 aa Nterminal acidic region, a 23 aa follistatinlike domain, a 55 aa kazallike segment and a 48 aa EFhand/calciumbinding domain. SPARCL1 is predicted at 75 kDa, but migrates at ~130 kDa, which has been explained either by disulfidelinked homodimerization or by glycosylation and high acidity. Some truncated forms have been reported. In mouse, a 55 kDa Cterminal fragment is the only form in kidney and represents a portion of SPARCL1 in other tissues. In humans, a 25 kDa form is increased in liver tumors that are encapsulated, while the fulllength form is downregulated in many epithelial cellderived tumors. SPARCL1 inhibits adhesion and spreading on a variety of substrate. It is thought to cause antiadhesive signaling that terminates neuronal migration, consistent with production by glial and neuronal cells during development or in response to trauma. In tonsillar high endothelial venules (HEV), SPARCL1 may induce endothelial cell dissociation, promoting extravasation. SPARCL1 binds collagen; in mice, deletion causes dermal collagen fibrils that are smaller in diameter and deficient in decorin. Human mature SPARCL1 shares 67%, 69%, 78%, 76%, 72% and 72% aa identity with mouse, rat, equine, canine, porcine and bovine SPARCL1, respectively. The follistatinlike, kazallike and calciumbinding domains of SPARCL1 show 61% aa identity with corresponding regions of SPARC